Matric Notes Class IX Biology Electron Microscope

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Electron Microscope

This is the most advanced form of microscope. Its resolving power is 250 times or more that of a compound microscope. In this microscope, a beam of electrons under high voltage is passed through the object and its image is reflected on to a screen through an electro magnetic lens to make a photograph.

With the help of microscope, any object an be magnified up to 250,000 its original size.

Comparison of Light Microscope and Electron Microscope

Light Microscope

• The radiation source is light so it is called light microscope.
• Wavelength of light is 400 – 700 nm.
• Maximum resolution is 200 nm.
• Maximum useful magnification is X 1500 with eye.
• Lenses are used.

Electron Microscope

• The radiation source is beam of electrons, so it is called Electron Microscope.
• Wavelength of beam of electrons is 0.005 nm.
• Maximum resolution is 0.5 nm.
• Maximum useful magnification is X. 250,000 on screen as image or photograph.
• Electromagnets are used.